Quick Diff Staining Procedure

ASA Institute
Procedure 4 1 dissolute Diff Staining Procedure
Semester jump off 2010
The Class Section MED 215-M08
Sojin Park
Dr. Victor Veloz

Introduction
The procedure that I am going to introduce is Quick Diff Staining Procedure. It is used for the derivative instrument count. After distinguishing 100 leukocytes, each of the five leukocytes are regarded as a percentage of the total 100 cells identified.
Definition of the procedure
Quick Diff Staining Procedure differentiates the distribution of the erythrocytes and five types of leukocytes on the priming of their characteristics, shapes, and sizes.
Materials
* Blood smear (On the sea-coast with having well-feathe carmine edge.)

* Alcohol Acetone (Fixative solution)
* eosin solution (fluorescent red dye)
* New methylene blue (Organic maculation agent)
* Staining rack (To place solutions)
* Bottled or running water system (To rinse exuberant solutions)
* Absorbent paper (To omit or absorb exuberant solutions)
Steps of the procedure
1. Dip the slide into the intoxicant acetone 3-5 times as the speed of second and remove the excessive solution with a paper towel.
2. Dip the slide into Eosin solution for 7 times and remove the excessive solution with a paper towel.
3. Dip the slide into late methylene blue for 9 times and remove the excessive solution with a paper towel.
4.



Use the incinerator to allow it to alter completely.
Result
* Erythrocytes: Pink or yellowish red.
* Platelets: majestic or color granules.
* Neutrophils: Blue nucleus, pink cytoplasm, violet granules.
* Eosinophils: Blue nucleus, blue cytoplasm, red granules.
* Basophils: Purple or dark blue nucleus, violet granules.
* Monocytes: Violet nucleus, light blue cytoplasm.
* Bacteria: Blue.
Reference range of WBC
* segmented neutrophil: 50 70 %
* Band neutrophil: 0 5%
* Lymphocyte: 20 40%
* Monocyte: 3 11%
* Eosinophil: 0 5%
* Basophil: 0 1%

Conclusion
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